Amanitin binding to calf thymus RNA polymerase B

Calf thymus polymerase.

The term polymerase has been applied to the deoxyribonucleoside triphosphate-polymerizing enzyme isolated from Escherichia coli (1). Enzymes with similar activity have been purified from regenerating rat liver (2) and calf thymus gland (3). An ample and varied supply of such polymerases is desirable for studies on the mechanism of deoxyribonucleic acid synthesis. The present work describes a pa...

Purification of RNA polymerase IIO from calf thymus.

Three subspecies of RNA polymerase II, designated IIO, IIA, and IIB, have been described in calf thymus and shown to differ in the apparent molecular weight of their largest subunits, designated IIo, IIa, and IIb, respectively. The objective of this study was to develop a procedure for the purification of RNA polymerase IIO. This form of the enzyme predominates in vivo and is responsible for th...

Oligodeoxyribonucleotide primers for calf thymus polymerase.

tion as measured by the hydroxamate reaction did not change appreciably, and furthermore, the amount of oxaloacetate synthesized was almost twice the amount of acetyl-CoA added. In other experiments enzymatic measurements of acetyl-CoA concentration gave similar results. The indirect role of acetyl-CoA is further confirmed by experiments shown in Table III. Oxaloacetate was formed from various ...

Novel poly(ADP-ribose) polymerase inhibitor veliparib: biophysical studies on its binding to calf thymus DNA

Veliparib (ABT-888), which can inhibit cancer growth by blocking DNA base excision repair, is one of several recently developed oral inhibitors of poly(ADP-ribose) polymerases, which are currently used in clinical trials. In this work, interaction of calf thymus DNA (ctDNA) with ABT-888 was first investigated following UV-visible absorption, nuclear magnetic resonance (NMR) spectroscopy, steady...

Size of the unwound region of DNA in Escherichia coli RNA polymerase and calf thymus RNA polymerase II ternary complexes.